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Olympus Fluoview 300 Confocal Laser Scanning Microscope

​​The Olympus Fluoview 300 is the newest addition to the facility. Our Confocal Laser Scanning Microscope​ (CLSM) is based on an Olympus BX50 upright microscope, and has Differential Interference Contrast (DIC) optics and two extra-long working distance water immersion objectives on a special two position turret. A microinjection system for electrophysiological studies is also available.
 
This microscope is used in the Bio 553 Confocal Microscopy class. It uses three PMT detectors, two for epi-confocal imaging, and one for transmitted-light imaging. Imaging capabilities include reflected-light confocal and transmitted-laser DIC imaging. Argon 488 nm and He/Ne lasers are installed on the microscope.
 
Objective lenses
4X, 10X, 20X, 60X Plan Apochromat DIC; 40X Plan Fluorite DIC; 20X Plan Fluorite Long Working Distance Phase; 20X and 40X Plan Fluorite Water-immersion DIC
 
Micromanipulators
Narishige hydraulic hanging joystick-controlled fine and coarse micromanipulators
 
Additional information

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