Allison Brookshier

Allison Brookshier
Mentor:  Dr. Deric Learman

Genomic Analysis of Chromium Resistant Bacteria
Microbial genomics is a fast growing field due to increases in technology making it possible to assemble and annotate genomes without extensive computation resources. The resulting genomic information can be used to understand how microbes can tolerate the broad environmental gradients (e.g. hydrothermal vents, human intestines, or soils) that they thrive in.  The environmental isolates were assembled using different genomic assemblers: Megahit, Spades, and IDBA UD. These assemblies were assessed for quality using QUAST and CheckM. Each genomic assembly was annotated to document the genes in each genome. The annotated genomes were used to determine the genes that aid the bacteria’s ability to resists heavy metals. This work can be used to help identify natural mechanisms where toxic chromium can be remediated in the environment.