Mentor: Dr. Mallory Wacker
Effectiveness of Differentiated THP-1 Cells as a Comparative Model for Macrophages
Immunology research typically utilizes the bodies specialized immune cells obtained from donors. This allows researchers the opportunity to directly work with the cells targeted within their research. The process of obtaining cells from donors is beneficial for precise results, but finding capable donors limits the amount of research that can be done. By differentiating THP-1 cells using Phorbol 12-myristate 13-acetate (PMA) working stock alongside a resting stage the ability of the differentiated cells to phagocytize bacteria and appear similarly to macrophages may be determinable. The overall aim of this experiment is to determine the effectiveness of differentiated THP-1 cells as a comparative model for macrophages.